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Since endotoxin (LPS) causes many of its effects indirectly by inducing cytokines, studies were undertaken to determine whether LPS might also induce splenic PTHrP expression
Soluble collagen in the supernatant was also analyzed by western blotting using an specific anti-collagen 1 type I antibody (sc-8784, Santa Cruz, Dallas, Texas) upon protein fractionation in sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) following protocols previously described 45
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Individuals following plant-based diets, such as vegans or vegetarians, may require more frequent injections due to lower baseline levels of naturally occurring Vitamin B12
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