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The samples were subsequently purified by size-exclusion chromatography using a Superose 6 10/300 column (Cytiva, Uppsala, Sweden) equilibrated with PBS (pH 7.5)

In general, supplementation with a broad range of micronutrients, rather than one or a selected few, would cover the micronutrient needs of more of the sample and would also be sensible given the well-known interactions among the various micronutrients with respect to cognition

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BPC-157 has a shorter half-life (approximately 4 hours) and is predominantly used in gastrointestinal and tendon research

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