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Quantification of immunoblots was performed using ImageJ (NIH)

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(2010) utilized IGF-1 and its analogs to investigate the relative contributions of PI3K/Akt versus MAPK/ERK signaling in IGF-1R-mediated cellular responses, demonstrating context-dependent pathway utilization across different cell types

They prevent excessive immune activation and suppress autoreactive T cells that would otherwise attack the body own tissues