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Sections were picked up on a 50-mesh copper grid (Ted Pella, G50) and were carbon-coated on both sides by a Cressington 208 carbon coater to prevent charging of the plastic, which can cause drift and thermal damage

van Straalen KR, Prens EP, Gudjonsson JE

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The N-terminal acetylation and C-terminal amidation are terminal modifications used in peptide analog design for experimental systems