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Cell plates were transferred to a LUMIstar plate reader (BMG Labtech) at 30 C for BRET measurements

Roque 2015)

Laser Ther

A vial touching the back wall can experience localized freezing that destroys the peptide in that portion of the solution

For inclusion in the group with T2D and a history of ASCVD, individuals also required a diagnosis of ASCVD at any time pre-index, broadly defined using International Classification of Diseases, Ninth Revision, Clinical Modification (ICD-9-CM) and ICD-10-CM codes (online Supplementary Table S1)