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Real-Time Quantitative Reverse Transcription PCR (qRTPCR) Analysis Total RNA from skin tissues was isolated using TRIzol reagent (Sigma, Milwaukee, WI, USA), and 1 g of total RNA was reverse-transcribed into cDNA
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Germ-free models, while mechanistically informative, lack normal immune development and may not fully reflect human physiology
2017;5:e13102
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