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Briefly, OPCs' extracts were prepared by resuspending cells in 0.1 M Tris-HCl (pH 8.0), 0.01 M EDTA, 0.05% Triton X-100 followed by sonication for 2 min with 30 s interval and centrifugation at 2,500 g, 4 for 5 min

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The transitions of the precursor ion to the product ion, cone voltage (V), and collision energy (eV) were listed (Supplementary Table 5)

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Since both drugs influence gastrointestinal function, starting with lower doses and gradual titration is generally advised