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Antibiotic mixture administered in NOD/ShiLt mice pre-EAE improved the disease course, correlated with enhanced Tregs and altered gut microbiota in Peyer's patches (Colpitts et al., 2017)
29(12):e340-e341
A dose that produces an effect in a mouse does not scale linearly to a human by body weight, and the research doses in this protocol are practitioner-derived conventions, not figures validated against human outcomes
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Step 2: Add 100ul HRP-Streptavidin (orange) into each well, seal the plate and static incubate for 30 minutes at 37C
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