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Peptides were then transferred to the gas phase using electrospray ionization, prefiltered by a FAIMS device (coefficient of variation 50 V) before entering the Orbitrap Exploris 480 (Thermo Fisher Scientific) mass spectrometer
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The substantially reduced IGFBP affinity makes IGF-1 DES a precise tool for in vitro studies requiring direct IGF-1R activation without IGFBP-mediated sequestration confounding the experimental readout
The notable increase in Cx32 level in hepatocytes of hepsin / mice, along with our previous work establishing hepsin as a regulator of the cellular abundance of hepatic GJ proteins (Hsu et al
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