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The results were analyzed by Student t-test and multivariate analysis of variance

The DNA sequence spanning the CBS gene ARE motif located 4970 bp upstream of the transcript start site and its mutants were cloned into a GFP expressing reporter pEGFP-N1 (PT3054, Clontech) with sequence specific primers (Supplementary Table 1)

Once phosphorylated, these transcription regulators could migrate into the nucleus of somatotroph cells, possibly impacting genes associated with growth hormone formation