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Example: Vial contains 5 mg (5000 mcg) MOTS-c Add 1 mL bacteriostatic water Concentration: 5000 mcg 1 mL = 5000 mcg/mL For 350 mcg per injection: 350 mcg 5000 mcg/mL = 0.07 mL or 7 units per injection Always use an insulin syringe for precise measurement

Cells were collected and washed with PBS once, and cellular fluorescence was determined by flow cytometry and data were collected by BD FACSCanto and analyzed with FlowJo V.10.1 software

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Another class of drugs that acts as a dual GLP-1/GIP (Glucose Dependent Insulinotropic Polypeptide) receptor agonist is called Tirzepatide, and a popular innovator drug of the class is Mounjaro