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The cells were kept in a humidified environment with 5% CO 2 at a temperature of 37 C
Fine lines and firmness over 8 to 12 weeks
Typical applications include glutathione S-transferase phase-II conjugation assays, glutathione peroxidase and glutathione reductase coupled assays, GSH/GSSG ratio determination as a redox readout in cultured cells, protein S-glutathionylation studies, and ferroptosis models where glutathione availability governs glutathione peroxidase 4 activity and therefore phospholipid hydroperoxide handling (Yang et al., Cell, 2014)
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