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Following the dehydration steps described above, slides were washed three to five times in RT diH 2 0 and then for 10 s in hot diH 2 0 to acclimate and subsequently incubated in hot 1 TRR (322000, ACD) for 5 min according to the manufacturers instructions

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Statistical analysis For each study population, the Semaglutide cohort and the comparison cohort were propensity-score matched (1:1 using nearest neighbor greedy matching with a caliper of 0.25 times the standard deviation) on covariates that are potential risk factors for CUD [42, 43] including demographics, adverse socioeconomic determinants of health (e.g., problems related to education and literacy, employment and unemployment, housing and economic circumstances, social environment, upbringing, primary support group including family circumstances and various psychosocial circumstances), problems with lifestyle (e.g., tobacco use, lack of physical exercise, inappropriate diet and eating habits, high-risk sexual behavior, gambling and betting, and other problems related to lifestyle including antisocial behaviors and sleep deprivation), pre-existing medical conditions and medications