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Gs recruitment assay G protein recruitment to CTR or individual AMYR subtypes was measured in COS-7 cells (ATCC, CRL-1651) transfected with c-myc-hCTRaleu-Rluc8:Gs-Venus:G1:G2, c-myc-hCTRaleu:RAMP1 Nluc:Gs-Venus:G1:G2, c-myc-hCTRaleu:RAMP2-Nluc:Gs-Venus:G1:G2 or c-myc-hCTRaleu:RAMP3-Nluc:Gs-Venus:G1:G2 at a ratio of 2:0.1:1:1:1, in a total of 51 ng DNA per well using PEI at a ratio of 1:6 of DNA to PEI
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A major extracellular enzyme, GPX3, is also found in the cytoplasm and plasma membranes of the mammalian kidney, epididymis, heart, lung, liver, brain, adipose tissue, mammary gland, and gastrointestinal tract (Nirgude and Choudhary, 2021)
Abstract Sea urchin grazing can drive kelp loss creating low productivity barrens with reduced biodiversity that may persist for decades
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The peptide itself is only one part of the equation
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