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Cells were cultured in Dulbeccos modified Eagles medium (DMEM) (Gibco, NY, USA) supplemented with 10 % foetal bovine serum (FBS) (Gibco, NY, USA) and were maintained in a humidified incubator at 37 C and 5 % CO 2

Vitamin D deficiency is extremely common, affecting an estimated 40% to 60% of the general population, and weight loss can further deplete stores since vitamin D is stored in fat tissue that is being reduced