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However, the high cost and frequent shortages of these FDA-approved drugs have led employers and patients to explore compounded GLP-1 medications as an alternative
RNA isolation and quantitative RT-PCR gene expression levels The total RNA from the kidney tissue was isolated using the TRIzol reagent (Invitrogen) [27], and the cDNA was synthesized following the manufacturers protocol, using 1 g RNA (Prime ScriptTM RT reagent Kit, Takara)
However, this frequency declines as they reach their objectives
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