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(2026a)
Immunoprecipitation Hepatocytes were lysed in an immunoprecipitation (IP) buffer (150 mM NaCl), 50 mM Tris-HCl, 1% Nonidet P-40, pH 7.8, and a mammalian cell-specific protease inhibitor cocktail
The deposition of callose in the plants was quantified using the method described by Pirelov et al
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In the RT structure, the cyano group at the position of the Michael acceptor enables fast reaction kinetics, the four-membered azetidine ring improves quantum yield and photostability 17 , and the two carboxylic acid groups ensure aqueous solubility and reduce probe binding to hydrophobic cellular structures
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