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In a comprehensive 2015 review published in BioMed Research International , Pickart and colleagues integrated three decades of preclinical and clinical research on GHK-Cu, reporting that the molecule stimulates collagen, elastin, glycosaminoglycan, and decorin synthesis in human dermal fibroblasts

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Transwell inserts allow the endothelial cells to grow on a membrane that sits in a stagnant tissue culture plate and is useful to measure the barrier properties of the subsequent monolayer