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The diluted genomic DNA was added to a 10 l reaction mix containing 0.2 mg/ml BSA, 4 mM DTT, 0.10% Tween, 0.1 mM dTTP, 1X phi29 buffer, and 15 U phi 29 DNA polymerase

Cycling Guidelines: Typical cycle length: 4 to 8 weeks Some users employ 4 weeks on, 2 to 4 weeks off protocols For acute injuries, shorter cycles of 2 to 4 weeks may be utilized Chronic conditions may warrant longer cycles under appropriate guidance How to Use / Administration Methods Subcutaneous Injection (Most Common) Subcutaneous injection into the fatty tissue of the abdomen, thigh, or near the injury site is the most widely used method