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Alternatively, when isotopic labeling is infeasible, unlabeled metabolomics and/or targeted biochemical assays can detect correlative changes in total off-target metabolite levels, while epistasis studies with multiple genetic and/or pharmacological manipulations can allow causal assessment of whether probable metabolic off-targets are required for given physiological or behavioral readouts

Without adequate glutathione, this process slows, and the liver's ability to clear accumulated toxins becomes compromised

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