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TLR3 expression was restricted to hFAPs whereas TLR7 , TLR8 , TLR9 , NOD2 , CLEC7A (Dectin-1), CLEC6A (Dectin-2) and CLEC4E (Mincle) were restricted to CD14 + monocytes

As previously mentioned, one of the hallmarks of ferroptosis is iron accumulation

In vitro analysis, using COS-7 cells [134] and motor neuron-like NSC-34 cells [135] has shown that mSOD1 can be secreted, and in vivo analysis appears to support these observations [134], but extracellular mSOD1 alone does not appear to be directly toxic to motor neurons [126]

The ggplot2 and pheatmap R software packages were conducted to visualize the DEGs by drawing volcano plots and heatmaps, respectively