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The concentrated solution was dialyzed with deionized water for 72 h, and then lyophilized to obtain D-P (yield: 90%)

Because of strict legislation on the use of cannabis, heroin, and amphetamines, NPS, especially from plants, has increasingly been used as alternatives by drug abusers

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To express SmTGR variants, 100 L of transformed BL21 (DE3) cells containing the corresponding plasmid were spread onto an LB agar plate (containing 25 g/mL kanamycin selection) and incubated for 1618 h at 37C