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Whilst the dynamic range of this assay was low in our hands, making it challenging to apply to higher throughput screening efforts or to concentration responses, it could be adapted by the use of alternative fluorophores with greater signal separation from the nanoluciferase emission peak (Dale et al ., 2019) or using complementation approaches (Inoue et al ., 2019)

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The trial was prematurely halted because it appeared unlikely to demonstrate any health benefit in supplemented patients about one-third of participants completed the trial at the time of study termination (75)