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Wheat plants were maintained in a growth chamber (L-41L2, Percival, Perry, IA) setting at 20 1C (day time) and 18 1C (night time) with a 14 h:10 h (L:D) photoperiod with light intensity of 1000 mol m 2 s 1
Niacinamide and adenosine round out the active profile, adding visible tone-evening and anti-aging support
Amplified single guide RNAs (sgRNAs) were quantified using the Illumina NovaSeq SP platform (Genomics Platform, Broad Institute)
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