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Formononetin treatment dose-dependently reduced mast cell degranulation, histamine release, and inflammatory cytokine expression in RBL-2H3 cells and mouse bone marrow derived mast cells, reducing IgE-induced MAPK and NF-B expression in vitro [122]

Estimation of folate levels were done under long term MTX treatment (2 nM and 10 nM, 3 days) where the macroscopic phenotypic traits were striking

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