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Rapamycin was administered to dogs at a dose of approximately 0.025 mg/kg, using 0.25 mg increments

Any abnormality in these molecules can cause an imbalance in mitochondrial dynamics (e.g., excessive mitochondrial fusion or fission), and can ultimately lead to disruptions in mitochondrial structure and function ( Mfn2 gene knockout in the mouse brain not only results in mitochondrial swelling, fragmentation, and structural disruption of mitochondrial cristae in hypothalamic and cortical neurons but also causes significant downregulation of ETC complexes (95)