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Before the experiment, the cells were loaded with 0.2% Fluo-4 AM, which was dissolved in HEPES-buffered Hanks Balanced Salt Solution (HBSS, Thermofisher) containing 1 mM CaCl2, 1 mM MgCl2, and 0.5% probenecid (Thermofisher) for one hour
They must first be placed in an FDA-cleared sharps container or heavy-duty plastic container, sealed securely, and then disposed of according to local regulations to prevent needlestick injuries
DOI: 10.1038/s41591-025-04102-x Cite This Page : ScienceDaily
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