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Cell plates were transferred to a LUMIstar plate reader (BMG Labtech) at 30 C for BRET measurements

Vitamin B6 levels can be checked with a blood test and if found to be low, can be corrected with oral supplementation

Species differences in receptor biology, metabolic rate, thermogenic capacity, pharmacokinetics, and peptide handling materially affect interpretation

Off-Target Activity Later animal research acknowledged the need to optimise the compound in relation to clearance and off-target activity against monoamine oxidase A

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