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Bioinformatics 25, 11891191
Cancer-associated fibroblasts (CAFs) have also been identified as active contributors to tumor progression in several solid malignancies [85] and have been shown to promote tumor growth by modulating iron metabolism [86]
Centrifuge the sample at 15,000 g at 4 C for 30 min, then dissolve the dry pellet in extraction buffer, thoroughly mixed by vortex and then placed in cold 0.1 M ammonium acetate - methanol solution for 12 h at 20 C
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