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Study design factors: This was a single-dose study at 5mg

Peptides were separated by chromatography with the following parameters: Acclaim PepMap100 C18 pre-column (2 cm, 75 m i.d., 3 m, 100 ), Pepmap-RSLC Proxeon C18 column (75 cm, 75 m i.d., 2 m, 100 ), 300 nl/min flow rate, using a gradient rising from 95% solvent A (water, 0.1% formic acid) to 40% B (80% acetonitrile, 0.1% formic acid) in 120 min, followed by a column regeneration of 20 min, for a total run of 140 min

Magnesium Citrate Unlike harsher laxatives, magnesium citrate supports peristalsisthe natural wave-like contractions of your intestines

Your blood volume decreases slightly as excess fluid is eliminated

You can reheat most meals in the microwave or oven (we used our Tovala oven), and soups are best heated on the stove