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For surface downregulation, labeling was performed with SNAP-Surface-488 in MIN6B1-SNAP-GLP-1R cells before fixation, or with anti-rodent GLP-1R (2 g l 1 ) in fixed MIN6B1 and INS-1 832/3 cells plus FITC-conjugated anti-mouse secondary (F0257, Sigma, 1/200)
This uncoupling of circulating glucocorticoid levels from tissue GR expression contrasts with the well-known in vitro effects of glucocorticoids in downregulating their own receptor (62)
Working with compounds that have established compounding pathways reduces uncertainty about legal and quality parameters
In summary, our results demonstrated that TG2 in cell lysates causes GST aggregate formation through crosslinking activity, which interferes with pulldown experiments and alters fused protein function
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