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In the RT structure, the cyano group at the position of the Michael acceptor enables fast reaction kinetics, the four-membered azetidine ring improves quantum yield and photostability 17 , and the two carboxylic acid groups ensure aqueous solubility and reduce probe binding to hydrophobic cellular structures

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Cysteine, the central amino acid in glutathione, contains a carbon atom that is bonded to a sulfur-hydrogen functional group

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