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The in vitro metabolic data established the half-life of AOD9604 in human serum and identified the primary metabolic pathways responsible for its clearance

After protein quantification, loading buffer containing 10% SDS, 0.5% bromophenol blue in Tris-HCL (pH 6.8), and 10% -mercaptoethanol (M3148, Sigma) was added to each cell lysate and boiled at 95-100 C for 10 min

Combination superior: 15-20% vs 5-10% weight loss

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Pen Dosage Chart Dosage & Protocols Variations Standard Research Protocol Dose: 0.1 0.3 mg (= 26 clicks) Duration: 2 4 weeks Frequency: Daily subcutaneous administration Cycle Interval: 2 weeks off before repeating Goal / Description: Baseline protocol for studying HPG axis activation and gonadotropin response