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Consensus guidelines on the use of intravenous ketamine infusions for chronic pain from the American Society of Regional Anesthesia and pain medicine, the American Academy of Pain Medicine, and the American Society of Anesthesiologists
Islet proteins were extracted using RIPA buffer (10 mM Tris-HCl, pH 7.4, 150 mM NaCl, 0.1% SDS, 1% Triton X-100, 1% sodium deoxycholate, 5 mM EDTA, 1 mM NaF, 1 mM sodium orthovanadate, protease inhibitors, and 125 M DPP-IV inhibitor) and quantified with the bicinchoninic acid protein assay kit (Pierce Chemical)
doi: 10.1038/s41594-023-01134-0 28 HeerspinkHJLPerkinsBAFitchettDHHusainMCherneyDZI