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Add bacteriostatic water to the lyophilized vial and the concentration is simply the vial's total mg divided by the water volume in mL

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was obtained from Energy Chemical (Shanghai, China)

Problem: you added the wrong amount of water You intended 1mL but realized after mixing that you added 2mL

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