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Co-culture experiments with PC3 WT and GGT1 + cell lines were performed using 6-well (24 mm) PET inserts with 0.4 m pores (VWR, 76313-902 or Corning, 354570)

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The next experimental step included extracting total RNA from the cells through the use of the acid guanidinium thiocyanate-phenol-chloroform extraction method