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According to FDA-approved prescribing information, unopened semaglutide pens must be refrigerated at temperatures between 36F and 46F (2C to 8C) until first use

Its sedative properties can be particularly beneficial in reducing pre-surgery jitters, allowing for a smoother transition into anesthesia

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Then, sections were incubated with rabbit anti-GLP-1R (1:50) at 4 C overnight and a secondary goat anti-rabbit antibody at 37 C was used for 30 min