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Restoring active glutathione (GSH) levels and the ratio of active reduce to inactive oxidized GSH/GSSG can correct some, if not all, of the energy depletion

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The MAPK and Akt inhibitors, UO126 and Akt inhibitor VIII respectively, were used to determine to what extent, if any, activation of their targets is required for Dihexa-mediated protection

After treatment, cells were immediately analyzed for apoptosis and the cell cycle using a BD FACSCelesta Cell Analyzer with an Annexin V-FITC/PI Apoptosis Detection Kit and PI/RNase Staining Buffer (BD Biosciences, Franklin Lakes, NJ, USA), respectively, according to the manufacturers instructions

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