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Coupling AlphaFold with FoldX identified conserved, nonactive site mutations in Fcy1, the 5-fluorocytosine target enzyme, that destabilize the protein, constituting a major mechanism of 5-FC resistance (Desprs et al., 2022)

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In vitro research found that metformin activated a novel galectin-directed ubiquitin signal transduction system in response to lysosomal membrane damage, induces autophagy, and facilitates the elimination of M.tb from macrophages [117]

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