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It was discovered in the 1990s by Croatian researchers studying gastric protective factors
The reaction was initiated by the addition of 190 l reaction buffer, adjusted to pH 7.4 by titration with K 2 HPO 4 (40 mM) and KH 2 PO 4 (10 mM) stock solutions, supplemented with NADH (100 M) and sodium pyruvate (600 M)
Glutathione gip ngn ch t thng do cc g t do gy ra v b v t bo kh h ho
The term GHK refers to the tripeptide glycyl-L-histidyl-L-lysine
A third section was used to stain acid-producing parietal cells of the corpus of the stomach by using an antibody directed against the H + K + ATPase -subunit (Abcam, Cambridge, UK
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