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The qPCR cycling conditions were as follows: an initial denaturation at 95 C for 15 min, followed by 30 cycles of 95 C for 7 s and 58 C for 10 s, and a final extension step at 95 C for 5 min, then 40 cycles of 95 C for 15 s and 58 C for 30 s

This can lead to less pigmentation, fewer dark spots, and a more even skin tone

Barth E, Stammler G, Speiser B, Schaper J

The effect plateaued by week 12, suggesting either receptor saturation or compensatory downregulationa detail with protocol implications for researchers designing extended-duration studies