arsenic glutathione Arsenic-Based Therapeutics The mechanisms of arsenic toxicity. – arsenic glutathione Nrf2 activation ameliorates

Herrera, C

Sau A, Pellizzari Tregno F, Valentino F, Federici G, Caccuri AM

Add 1,000 L (1 mL) of reconstitution solvent to the 1 mg vial This yields a 1 mg/mL (1,000 g/mL) stock solution To achieve 100 g/mL working stock: Dilute 100 L of 1 mg/mL stock into 900 L of PBS or cell culture medium yields 100 g/mL in 1 mL To achieve 10 ng/mL in a 5 mL experiment: Take 0.5 L of 100 g/mL working stock + 4,999.5 L of medium Alternatively: dilute to an intermediate concentration to reduce pipetting error Use a dilution series approach (stock working stock final experimental concentration) to minimize pipetting inaccuracy at nanogram-level concentrations

To gauge improvement, track specific issues you had before, for instance, frequency of mouth sores (which can occur with B deficiencies), or numbness in feet, or level of fatigue on a 10-point scale

Science has found ways to restore this balance