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The second step is catalyzed by GSH synthase, which adds glycine to -glutamylcysteine to form -glutamylcysteinylglycine or GSH

Examples include (i) antiapoptotic transcriptional activity triggered by persulfide-mediated binding of ribosomal protein S3 (RPS3) to nuclear factor kappa-light-chain-enhancer of activated B cells (NF-B) ( 35 ), (ii) extracellular signalregulated kinases 1/2 (ERK1/2) translocation into the nucleus facilitated by mitogen-activated protein kinase kinase (MEK1) persulfide formationinduced poly(ADP-ribose) polymerase 1 (PARP-1) activation leading to DNA damage repair ( 36 ), (iii) adenosine triphosphate (ATP)sensitive channel activation upon persulfide-induced inhibition of ATP binding ( 37 ), and (iv) potent inhibition of PTEN ( 23 ) or of protein-tyrosine phosphatase 1B (PTP1B) ( 20 ) activities by persulfidation of their active-site Cys residues

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For cAMP and receptor expression assays, HEK293T cells were seeded into 6-well cell culture plates at a density of 7 10 5 cells per well