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Genetic susceptibility to autoimmune thyroid disease: past, present, and future

coli containing plasmid pANT35 or pANT62 was inoculated from an overnight culture at a ratio of 1:100 in a 2 l baffled Erlenmeyer flask filled with 400 ml Zym-5052 medium 51 containing 50 g ml 1 kanamycin sulfate per flask, incubated at 20 C, 140 rpm, 5 cm shaking diameter for 24 h, and the final OD 600 was determined using a CO8000 cell density meter (WPA biowave)

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doi:10.1002/nbm.4227 112. Shimizu, T., Iwanaga, M., Yasunaga, A., Urata, Y., Goto, S., Shibata, S., & Kondo, T

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