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1 ug of RNA was reverse transcribed using the Superscript III Reverse Transcriptase kit (Invitrogen) according to the manufacturers instructions

10 AP2 expression persists until E12.5 during PGC development, 10 suggesting that AP2 may function after germ cell specification

140 Scopoleitin mitigates APAP-induced hepatotoxicity through suppression of NLRP3 gene expression and concurrent inhibition of the Nrf2/HMGB1/TLR4/NF-B signaling pathway, resulting in reduced levels of proinflammatory factors

NaOH (6.25 mmol) and chloroacetic acid (0.250 g, 11.655 mmol) was then added

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