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The assay plate was incubated for 3 h at 37 C in 5% CO 2 and allowed to equilibrate at room temperature for 5 min after which 30 L SteadyLite Plus (PerkinElmer, 6066759) was added to each well

(Basel, Switz.) 25 (18), 4320

Lansley et al

For this study, a nested casecontrol design was employed in which patients were selected based on their overweight/obesity status and control subjects were age- and sex-matched subjects, both recruited at a ratio of 1:1.6

Characteristics and Research Mechanism Peptides in this class are used in modern research to investigate cellular receptors, peptide signaling, and metabolic pathways