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The following formula was used to calculate the rate of tissue volumetric shrinkage 44 : where AA and AB were the areas of circular pieces, after and before tissue processing, cutting, and staining, respectively (AB = 4.5 mm)
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Journal of Medicinal Chemistry
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Various studies have been performed recently too, such as this one by the US National Library of Medicine, but there is no conclusive evidence of it working for skin lightening yet
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