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Here, the concentration of DTT in the binding buffer used for preparing the tracerE3 ligase mixture varied from 0.1 to 5 mM

doi:10.4103/0974-7796.75862 [60] Miller C, Emmanuel A, Zarate-Lopez N, Taylor S, Bloom S

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The light source is HeNe laser (l = 633 nm), 22 mW output power