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They received instructions to swallow it three times a day ( three capsules of 1 g each), after main meals, recording daily the quantity of capsules ingested on a specific form named Participants daily card. For each return visit to the site of research, aiming to receive, the participants answered to an adherence form, filled out by the pharmacist, immediately after medical appointment

Cultivation conditions: Aerobic and anaerobic bacteria media (such as thioglycolate fluid media) were incubated at 30-35 for 14 days