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qPCR was performed on a ViiA7 real-time PCR system (Applied Biosystems) with SYBR green master mix (Vazyme, Q711-02), as described previously 51

Biochem Biophys Res Commun 231:488493 Aureli T, Puccetti C, Di Cocco ME, Arduini A, Ricciolini R, Scalibastri M, Manetti C, Conti F (1999) Entry of [(1,2-13C2)acetyl]-l-carnitine in liver tricarboxylic acid cycle and lipogenesis: a study by 13 C NMR spectroscopy in conscious, freely moving rats

Eating any further feels quite uncomfortable and you feel stuffed

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